Recombinant 170 kd subunit lectin of entamoeba histolytica...

Details Recombinant 170 kd subunit lectin of entamoeba histolytica... Recombinant 170 kd subunit lectin of entamoeba histolytica...

C - Chemistry, Metallurgy

12

N

C12N 15/30 (2006.01) A61K 39/002 (2006.01) C07K 14/44 (2006.01) G01N 33/569 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2165202

Recombinantly produced 170 kDa heavy subunit of Entamoeba histolytica Gal/GalNAc adherence lectin or an epitope-bearing portion thereof may be used as antigen in serological analysis of E. histolytica infection or as a vaccine for immunizing against infection. The recombinant production is achieved in a procaryotic system to provide non-glycosylated antigens or immunogens which are immunologically reactive. Also disclosed are three genes which encode epitope-bearing portions of the lectin.

Une sous-unité lourde de 170 kDa produite par recombinaison de lectine d'adhésion Gal/GalNAc d'Entamoeba histolytica ou une partie porteuse d'épitopes de celle-ci peut être utilisée en tant qu'antigène dans l'analyse sérologique de l'infection par E.histolytica ou en tant que vaccin d'immunisation contre l'infection. La production par recombinaison s'effectue dans un système procaryote afin de produire des antigènes ou des immunogènes non glycosylés immunologiquement réactifs. Trois gènes codant les parties porteuses d'épitopes de la lectine sont également décrits.

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