Recombinant adeno-associated virus vectors

Details Recombinant adeno-associated virus vectors Recombinant adeno-associated virus vectors

C - Chemistry, Metallurgy

12

N

C12N 15/86 (2006.01) C07K 14/015 (2006.01) C07K 14/05 (2006.01) C12N 7/04 (2006.01) C12N 15/38 (2006.01) C12N 15/864 (2006.01) C12N 15/869 (2006.01)

Patent

CA 2054517

A simplified method to produce recombinant adeno-associated virus (AAV) vectors is described. The procedure involves the use of chimeric plasmids which incorporate the Epstein Barr nuclear antigen (EBNA) gene, the latent origin of replication of Epstein Barr Virus (oriP), and a recombinant AAV genome. The chimeric plasmids themselves are also a part of the present invention. These EBV/AAV plasmids are maintained as multicopy extra-chromosomal elements in cells, such as human 293 cells. Permanent cell lines carrying these EBV/AAV plasmids are induced to produce large amounts of recombinant AAV virus upon addition of wild-type, adeno-associated virus helper functions. Recombinant AAV vectors produced in this manner are capable of transducing exogenous genes into other human cell lines and exhibit all of the attributes of viral elements produced by conventional methods.

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