C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/50 (2006.01) C12N 9/88 (2006.01)
Patent
CA 2401315
The invention concerns a recombinant adenylcyclase, comprising at least a polypeptide sequence including one or several cleavage site of at least a molecule with site-specific proteolytic activity, said polypeptide sequence being inserted in the catalytic domain of an adenylcyclase while preserving its enzymatic activity. The invention also concerns methods for screening molecules with proteolytic activity using said recombinant adenylcyclase.
La présente invention concerne une adénylcyclase recombinante, comprenant au moins une séquence polypeptidique incluant un ou plusieurs sites de clivage d'au moins une molécule à activité protéolytique site-spécifique, ladite séquence polypeptidique étant insérée dans le domaine catalytique d'une adénylcyclase tout en conservant son activité enzymatique. L'invention concerne également des procédés de tri de molécules à activité protéolytique utilisant cette adénylcyclase recombinante.
Dautin Nathalie
Karimova Gouzel
Ladant Daniel
Ullmann Agnes
Centre National de La Recherche Scientifique (cnrs)
Institut Pasteur
Ogilvy Renault Llp/s.e.n.c.r.l.,s.r.l.
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