Recombinant alveolar surfactant protein

C - Chemistry – Metallurgy – 12 – N

Patent

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167/103, 195/1.2

C12N 15/12 (2006.01) A61K 35/42 (2006.01) A61K 38/17 (2006.01) A61K 47/24 (2006.01) C07K 14/785 (2006.01) A61K 38/00 (2006.01)

Patent

CA 1340611

The complete coding sequences and amino acid sequences for both canine and human 1OK alveolar surfactant proteins (ASP) are disclosed; clones encoding variants of the SP-18 and SP-5 forms of human protein are disclosed. Methods and vectors for obtaining these proteins in recombinant form are also described. An improved method for purification of the 32K protein takes advantage of its carbohydrate affinity. Pharmaceutical compositions in the treatment of respiratory deficiency syndromes use the 1OK proteins with or without the 32K form.

535980

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