Recombinant bicistron adenovirus for treating pathological...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/86 (2006.01) A61K 48/00 (2006.01) C07K 14/47 (2006.01) C12N 9/10 (2006.01) C12N 9/20 (2006.01) C12N 15/861 (2006.01)

Patent

CA 2271437

The invention concerns a defective recombinant virus and preferably an adenovirus characterised in that it comprises at least two nucleic acids coding for distinct enzymes, proteins and/or co-factors involved in the reverse transfer of cholesterol, said nucleic acids being operationally bound to a transcriptional promoter and mutually separated by a sequence coding for an internal entry site of the IRES ribosome. The invention further concerns plasmid constructs useful for preparing these adenovirus, and cells transformed by these plasmids or adenovirus and pharmaceutical compositions containing said adenovirus.

La présente invention concerne un virus recombinant défectif et de préférence un adénovirus caractérisé en ce qu'il comprend au moins deux acides nucléiques codant pour des enzymes, protéines et/ou co-facteurs distincts et impliqués dans le transport inverse du cholestérol, lesdits acides nucléiques étant liés opérationellement à un promoteur transcriptionnel et séparés l'un de l'autre par une séquence codant pour un site d'entrée interne du ribosome IRES. Elle se rapporte en outre à des constructions plasmidiques utiles pour préparer ces adénovirus, à des cellules transformées par ces plasmides ou adénovirus et à des compositions pharmaceutiques contenant lesdits adénovirus.

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