Recombinant c-terminal .alpha.-amidating enzyme derivative

Details Recombinant c-terminal .alpha.-amidating enzyme derivative Recombinant c-terminal .alpha.-amidating enzyme derivative

C - Chemistry, Metallurgy

12

N

C12N 15/12 (2006.01) C12N 1/21 (2006.01) C12N 9/02 (2006.01)

Patent

CA 2691374

Disclosed are: a C-terminal-.alpha.-amidated recombinant enzyme derivative which lacks the formation of at least one disulfide bond among five disulfide bonds occurring in a C-terminal-.alpha.- amidated enzyme derived from Xenopus laevis; DNA encoding the derivative; an expression vector carrying the DNA; a bacterium Escherichia coli transformed with the expression vector; and a method for producing the derivative by using the bacterium Escherichia coli.

L'invention concerne : un dérivé d'enzyme recombinante C-terminal-a-amidée auquel manque la formation d'au moins une liaison disulfure parmi les cinq liaisons disulfures présentes dans une enzyme C-terminal-a-amidée dérivée de Xenopus laevis; un ADN codant pour le dérivé ; un vecteur d'expression qui porte l'ADN ; une bactérie Escherichia coli transformée avec le vecteur d'expression ; et un procédé de fabrication du dérivé en utilisant la bactérie Escherichia coli.

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