Recombinant chimeric virus

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 7/01 (2006.01) A61K 39/245 (2006.01) A61K 39/255 (2006.01) A61P 31/22 (2006.01) A61P 37/04 (2006.01) C12N 15/19 (2006.01) C12N 15/38 (2006.01) C12N 15/45 (2006.01) C12N 15/86 (2006.01) C12N 15/869 (2006.01)

Patent

CA 2626498

This invention provides a recombinant herpesvirus of turkeys comprising a foreign DNA sequence encoding a cytokine inserted into an insertion region which comprises a XhoI site within an EcoR1 #9 fragment of a herpesvirus of turkeys viral genome, and the foreign DNA sequence encoding a cytokine which is capable of being expressed in a host cell infected with the herpesvirus of turkeys. This invention provides a recombinant herpesvirus of turkeys-Marek's disease virus chimera comprising a herpesvirus of turkeys unique long viral genome region and a Marek's disease virus unique short region. Lastly, this invention provides homology vectors for producing a recombinant herpesvirus of turkeys, host cells, and vaccines and methods for immunization.

L'invention concerne un virus recombinant de l'herpès du dindon comportant une séquence d'ADN étranger codant pour une cytokine insérée dans une région d'insertion qui contient un site XhoI dans un fragment EcoR1 #9 du génome viral du virus de l'herpès du dindon ainsi qu'une séquence d'ADN étranger codant pour une cytokine pouvant être exprimée dans une cellule hôte infectée par le virus de l'herpès du dindon. Cette invention porte également sur un virus chimère recombinant de l'herpès du dindon et de la maladie de Marek constitué d'une longue région unique du génome du virus de l'herpès du dindon et d'une courte région unique du virus de la maladie de Marek. Cette invention fournit aussi des vecteurs d'homologie permettant de mettre au point un virus recombinant de l'herpès du dindon, des cellules hôtes, des vaccins et des méthodes d'immunisation.

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