Recombinant co-expression of vitamin k epoxide reductase...

C - Chemistry – Metallurgy – 12 – N

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C12N 9/04 (2006.01)

Patent

CA 2599284

The present invention relates to a host organism containing recombinant nucleic acids coding for the vitamin K reductase complex subunit 1 (VKORC1) and recombinant nucleic acids coding for a vitamin K dependent (VKD) protein, wherein both the recombinant VKORC1 and the recombinant VKD protein are expressed in said host organism. Further, the present invention relates to a cell culture system comprising cells which contain said recombinant nucleic acids and to methods for improving the productivity of recombinant VKD protein expression in a host organism being cultured in suitable systems.

La présente invention concerne un organisme hôte contenant un codage d~acides nucléiques de recombinaison pour l~unité auxiliaire 1 du complexe de réductase de la vitamine K (VKORC1) et un codage d~acides nucléiques de recombinaison pour une protéine liée à la vitamine K (VKD), la VKORC1 de recombinaison et la protéine VKD de recombinaison étant toutes deux exprimées dans ledit organisme hôte. En outre, la présente invention concerne un système de culture cellulaire comprenant des cellules contenant lesdits acides nucléiques de recombinaison et des procédés pour améliorer la productivité d~une expression de protéine VKD de recombinaison dans un organisme hôte en culture dans des systèmes adéquats.

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