Recombinant constructs for use in reducing gene expression

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/82 (2006.01)

Patent

CA 2567087

Recombinant constructs useful for reducing the expression of at least one target nucleic acid fragment of interest and any substantially similar endogenous nucleic acid fragment of interest are disclosed. In particular, a recombinant construct comprising at least one nucleic acid of interest is inserted between two convergent promoters. A plant or plant organ stably transformed with this construct will have a reduction in expression of the target nucleic acid fragments of interest.

L'invention concerne des constructions recombinantes utiles pour diminuer l'expression d'au moins un fragment d'acide nucléique cible digne d'intérêt et tout fragment d'acide nucléique endogène sensiblement similaire digne d'intérêt. Plus spécifiquement, l'invention concerne une construction recombinante comprenant au moins un acide nucléique digne d'intérêt, laquelle est insérée entre deux promoteurs convergents. Une plante ou un organe de plante transformés de façon stable avec cette construction présentera une diminution de l'expression des fragments d'acide nucléique cibles dignes d'intérêt.

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