C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/1.14, 195/1.
C12N 15/70 (2006.01) C12N 9/78 (2006.01) C12N 15/66 (2006.01)
Patent
CA 2015046
The recombinant DNA according to the present invention contains a regulator region which is at least 50 % homologous to the sections -969 to -991 base pairs and/or -1308 to -1330 base pairs of the sequence in Fig. and in the 3' direction from the regulation sequence contains at least one promoter region which in turn contains a -35/-10 promoter sequence. An expression vector according to the present invention contains a recombinant DNA according to the present invention ligated into a suitable vector molecule. To prepare the recombinant DNA according to the present invention, the pfl gene with its upstream regions is isolated from the gene bank of a microorganism, the desired parts are obtained according to well-known methods and they are combined with the other desired sequences or promoters. When using a recombinant DNA according to the present invention or an expression vector according to the present invention for the inducible and the repressible expression of a foreign gene, the induction is effected under anaerobic conditions and by pyruvate and the repression is effected by oxygen.
Bock August
Herbst Roland
Jarsch Michael
Sawers Robert G.
Bock August
Herbst Roland
Jarsch Michael
Ogilvy Renault Llp/s.e.n.c.r.l.,s.r.l.
Roche Diagnostics Gmbh
LandOfFree
Recombinant dna and expression vector does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Recombinant dna and expression vector, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Recombinant dna and expression vector will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1523229