Recombinant dna and process for the production of chimeric...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/13 (2006.01) C07K 16/28 (2006.01) C07K 16/46 (2006.01) C12P 21/02 (2006.01) C12P 21/08 (2006.01)

Patent

CA 2043516

A b s t r a c t A recombinant DNA which codes for the light or heavy chain of an antibody whose constant regions are of human origin and whose variable regions are of non-human origin, and which has in the direction of transcription (a) a cDNA sequence which codes for the variable, non- human region, including the region J and, if desired, D, (b) an intron sequence which has a splice donor site at its 5' end and is composed of a non-human intron partial sequence at the 5' end and a human intron partial sequence at the 3' end and (c) a genomic DNA sequence coding for the human constant region.

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