Recombinant dna constructs and methods for modulating...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/82 (2006.01) C12N 15/113 (2010.01) C12N 5/10 (2006.01)

Patent

CA 2729713

This invention provides recombinant DNA constructs and methods for manipulating expression of a target gene that is regulated by a small RNA, by interfering with the binding of the small RNA to its target gene. More specifically, this invention discloses recombinant DNA constructs encoding cleavage blockers, 5-modified cleavage blockers, and translational inhibitors useful for modulating expression of a target gene and methods for their use. Further disclosed are miRNA targets useful for designing recombinant DNA constructs including miRNA-unresponsive transgenes, miRNA decoys, cleavage blockers, 5-modified cleavage blockers, and translational inhibitors, as well as methods for their use, and transgenic eukaryotic cells and organisms containing such constructs.

La présente invention concerne des constructions d'ADN recombinant et des procédés pour manipuler l'expression d'un gène cible qui est régulée par un petit ARN, en interférant sur la liaison du petit ARN avec son gène cible. Plus particulièrement, cette invention concerne des constructions d'ADN recombinant codant pour des bloqueurs du clivage, des bloqueurs du clivage modifiés en position 5, et des inhibiteurs de la traduction utiles pour moduler l'expression d'un gène cible et des procédés pour leur utilisation. L'invention concerne en outre des cibles d'ARNmi utiles pour concevoir des constructions d'ADN recombinant comprenant des transgènes insensibles à l'ARNmi, des leurres d'ARNmi, des bloqueurs du clivage, des bloqueurs du clivage modifiés en position 5, et des inhibiteurs de la traduction, ainsi que des procédés pour leur utilisation, et des cellules eucaryotes transgéniques et des organismes contenant de telles constructions.

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