C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/1.22, 195/1.
C12N 15/52 (2006.01) C07H 21/04 (2006.01) C12N 5/10 (2006.01) C12N 9/00 (2006.01) C12N 15/69 (2006.01) C12N 15/70 (2006.01) C12N 15/74 (2006.01) C12N 15/80 (2006.01) C12N 15/90 (2006.01)
Patent
CA 1309675
X-6932 Abstract The present invention comprises novel DNA com- pounds that encode isopenicillin N synthetase. The invention also comprises methods, transformants, and polypeptides related to the novel DNA compounds. The novel isopenicillin N synthetase-encoding DNA, together with its associated transcription and translation activating sequence, was isolated from Penicillium chrysogenum. The isopenicillin N synthetase-encoding DNA can be used to construct novel E. coli expression vectors that drive expression of isopenicillin N syn- thetase in E. coli. The intact P. chrysogenum iso- penicillin N synthetase-encoding DNA and associated transcription and translation activating sequence can also be used to construct expression vectors that drive expression of the isopenicillin N synthetase in P. chrysogenum and Cephalosporium acremonium. The transcription and translation activating sequence can be fused to a hygromycin phosphotransferase-encoding DNA segment and placed onto expression vectors that function in P. chrysogenum and C. acremonium. The transcription termination and mRNA polyadenylation signals of the P. chrysogenum isopenicillin N synthetase can be used to increase ultimate expression of a product encoded on a recombinant DNA vector.
523432
Carr Lucinda Gayle
Ingolia Thomas Dominick
Queener Stephen Wyatt
Skatrud Paul Luther
Eli Lilly And Company
Gowling Lafleur Henderson Llp
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