C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/39, 195/1.14
C12N 15/54 (2006.01) C12N 1/15 (2006.01) C12N 1/21 (2006.01) C12N 9/00 (2006.01) C12N 9/10 (2006.01) C12N 15/69 (2006.01) C12N 15/80 (2006.01)
Patent
CA 1309673
Abstract The present invention comprises novel DNA com- pounds that encode isopenicillin N synthetase and also comprises related methods, transformants, and polypeptides. The novel isopenicillin N synthetase-encoding DNA, together with its associated transcriptional and translational activating sequence, was isolated from Cephalosporium acremonium and cloned into an E. coli cloning vector. The isopenicillin N synthetase-encoding DNA has been used to construct novel E. coli expression vectors that drive expression of a stable, active, and novel isopeni- cillin N synthetase in E. coli. The intact C. acremonium isopenicillin N synthetase-encoding DNA and associated transcriptional and translational activating sequence have also been used to construct C. acremonium expression vectors that drive expression of the isopenicillin N synthetase in C. acremonium. The C. acremonium transcrip- tional and translational activating sequence has further been fused to a hygromycin phosphotransferase-encoding DNA segment and placed onto C. acremonium expression vectors. Useful derivatives of the novel compounds and vectors are also described.
506967
Ingolia Thomas Dominick
Queener Stephen Wyatt
Samson Suellen Mary
Skatrud Paul Luther
Eli Lilly And Company
Gowling Lafleur Henderson Llp
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