Recombinant-dna mediated production of xanthan gum

Details Recombinant-dna mediated production of xanthan gum Recombinant-dna mediated production of xanthan gum

C - Chemistry, Metallurgy

12

N

195/140, 195/1.2

C12N 15/52 (2006.01) C12N 1/21 (2006.01) C12P 19/04 (2006.01) C12P 19/06 (2006.01)

Patent

CA 1338138

Methods for the recombinant-DNA mediated production of xanthan gum and gum variants structurally related to xanthan are disclosed. The methods in part involve the synthesis of these polysaccharides in anaerobic and/or denitrifying hosts. In particular, plasmids pX209 and pRK290-H366 are disclosed which contain the genes, isolated from X. campestris, encoding Transferase I, Transferase II, Transferase III, Transferase IV, Transferase V, Ketalase, Acetylase and Polymerase. These plasmids have been deposited in the American Type Culture Collection under Accession Nos. 67051 and 67049, respectively. More particularly, the methods involve (a) obtaining a vector containing a nucleic acid sequence encoding enzymes for the synthesis of xanthan polysaccharide or a xanthan variant polysaccharide, (b) transferring the vector to a host organism, (c) culturing the organism under conditions appropriate for synthesis of the xanthan polysaccharide or variant xanthan polysaccharide, and (d) harvesting the polysaccharide.

532837

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