Recombinant dna method and host cells

C - Chemistry – Metallurgy – 12 – N

Patent

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195/1.29, 195/1.

C12N 15/67 (2006.01) C12N 1/19 (2006.01) C12N 5/10 (2006.01) C12N 15/85 (2006.01) C12P 21/00 (2006.01)

Patent

CA 2024607

2024607 9008191 PCTABS00001 The present invention provides a process and host cell for use in increasing the amount of a desired protein produced by a cell line. The process and the cell line use a first transcription unit containing a gene for a transactivator protein to control the transactivation of a second transcription unit such that the amount of desired protein expression can be increased without affecting adversely cell growth. Preferred transactivatior proteins are derived from E1A.

La présente invention concerne un procédé et une cellules hôte permettant d'augmenter la production d'une protéine voulue par une lignée de cellules. Ledit procédé et la lignée de cellule utilisent une première unité de transcription contenant un gène pour une protéine de transactivation, qui commande la transactivation d'une seconde unité de transcription, de sorte que l'on peut augmenter la quantité d'une expression de protéine voulue sans affecter de manière contraire la croissance des cellules. Les protéines de transactivation sont de préférence dérivées du gène E1A.

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