Recombinant dna methods, vectors and host cells

Details Recombinant dna methods, vectors and host cells Recombinant dna methods, vectors and host cells

C - Chemistry, Metallurgy

12

N

195/1.2, 195/1.2

C12N 15/85 (2006.01) C12N 5/10 (2006.01) C12N 9/00 (2006.01) C12N 9/72 (2006.01) C12N 15/13 (2006.01) C12N 15/52 (2006.01) C12N 15/67 (2006.01)

Patent

CA 1338891

The present invention relates to a method for transforming a lymphoid cell line to glutamine independence which comprises: transforming the lymphoid cell line with a vector containing an active glutamine synthetase (GS) gene; growing the transformed cell line on a medium containing glutamine; and continuing the growth of the transformed cell line on a medium in which the glutamine is progressively depleted or on a medium lacking glutamine, and to vectors and host cells for use in the method.

597034

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