Recombinant dna plasmid for xanthan gum synthesis

Details Recombinant dna plasmid for xanthan gum synthesis Recombinant dna plasmid for xanthan gum synthesis

C - Chemistry, Metallurgy

12

N

195/1.14, 195/1.

C12N 15/01 (2006.01) C12N 1/21 (2006.01) C12N 15/52 (2006.01) C12P 19/06 (2006.01)

Patent

CA 1286239

TITLE OF INVENTION RECOMBINANT DNA PLASMID FOR XANTHAN GUM SYNTHESIS ABSTRACT OF THE DISCLOSURE Described is a DNA fragment containing some essential genetic material for xanthan gum synthesis. The fragment is isolated from gum-producing Xanthomonas campestris and inserted into a cloning vector using recombinant DNA technology. A resulting recombinant plasmid, pCHC3, restores xanthan gum synthesis to non-xanthan gum forming (non-mucoid) mutants, increases xanthan gum yield in gum-producing strains by gene augmentation, and increases the pyruvate content of the obtained xanthan gum. The invention encompasses other DNA fragments isolated from gum-producing X. campestris and which, after appropriate manipulations, restore xanthan gum synthesis in substantially the same fashion as the fragment of pCHC3.

529171

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