Recombinant dna production of beta-1, 3-glucanase

Details Recombinant dna production of beta-1, 3-glucanase Recombinant dna production of beta-1, 3-glucanase

C - Chemistry, Metallurgy

12

N

C12N 15/56 (2006.01) C12N 1/21 (2006.01) C12N 9/24 (2006.01) C12N 15/72 (2006.01)

Patent

CA 2069962

2069962 9203557 PCTABS00011 The present invention relates to recombinant .beta.-1,3-glucanase essentially free of proteases. The enzyme is obtained through the use of a recombinant DNA expression vector which comprises a DNA sequence encoding the .beta.-1,3-glucanase gene or mutants and variants thereof placed under the control of an exogenous expression promoter, preferably a bacterial promoter. Also, the .beta.-1,3-glucanase gene may include sequences flanking the open reading frame of the native .beta.-1,3-glucanase gene. The present invention also relates to a microorganism transformed with a recombinant DNA expression vector comprising the .beta.-1,3-glucanase gene or mutants and variants thereof under the control of an exogenous expression promoter.

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