Recombinant dna techniques

Details Recombinant dna techniques Recombinant dna techniques

C - Chemistry, Metallurgy

12

N

530/5.1, 530/15.

C12N 1/20 (2006.01) C07H 21/00 (2006.01) C07K 5/072 (2006.01) C12N 15/00 (2006.01) C12P 13/20 (2006.01) C12P 13/22 (2006.01) C12P 21/00 (2006.01) C12P 21/02 (2006.01)

Patent

CA 1189466

ABSTRACT A synthetic gene which comprises, in the coding strand, codons for the amino acids of a desired peptide in tandem repeat is disclosed. A process for the production of such a synthetic gene which comprises the self-assembly and ligation and optionally repair of a plurality of appropriate oligonucleotides, the assembly to give the repeating character being achieved by self-complementary overlapping ends at the termini of the group of oligo- nucleotides which when assembled, comprises the repeating unit of the gene is also disclosed. Such synthetic genes may be used to produce recombinant plasmid vectors, to transform cell and hence to express peptides. Inter alia the present invention may be applied to the production of (aspartyl-phenylalanine)n and hence the sweetener "Aspartame".

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