Recombinant dna viral vector for transfecting animal cells

Details Recombinant dna viral vector for transfecting animal cells Recombinant dna viral vector for transfecting animal cells

C - Chemistry, Metallurgy

12

N

C12N 15/861 (2006.01) A61K 48/00 (2006.01) C12N 9/00 (2006.01) C12N 15/86 (2006.01)

Patent

CA 2157063

An animal cell is co-transfected with both a recombinant DNA viral vector which bears a promoter, a recombinase gene and a poly(A) sequence and a recombinant DNA viral vector which bears two recombinase-recongnizing sequences and which further bears an origin of replication, a promoter, a foreign gene and a poly(A) sequence, each of which is located between the two recombinase-recongnizing sequences. Thereafter, in the co-transfected animal cell, a DNA fragment containing the origin of replication, promoter, foreign gene and poly(A) sequence is excised from the vector by the action of a recombinase expressed in the another vector. The DNA fragment forms a circular DNA molecule which autonomously replicates in the co-transfected animal cell due to the origin of replication, whereby the foreign gene is continuously expressed. Accordingly, the combination of the above two DNA viral vectors wherein a gene which is defective in patients is used as the foreign gene is quite useful especially for the treatment of the patient with hereditary diseases.

Affiliated with

Also associated with

Rating

Recombinant dna viral vector for transfecting animal cells does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Recombinant dna viral vector for transfecting animal cells, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Recombinant dna viral vector for transfecting animal cells will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1661332