Recombinant enzymatically active calpain expressed in a...

Details Recombinant enzymatically active calpain expressed in a... Recombinant enzymatically active calpain expressed in a...

C - Chemistry, Metallurgy

12

N

C12N 9/64 (2006.01) C12N 5/10 (2006.01) C12N 7/01 (2006.01) C12N 15/57 (2006.01) C12N 15/86 (2006.01)

Patent

CA 2194763

The present invention is directed to mammalian enzymatically active calpain produced in insect cells by recombinant means. Recombinant vectors and baculoviruses containing cDNA encoding the 80 kDa subunit, 30 kDa subunit, and both subunits are described. Methods for producing recombinant enzymatically active mammalian calpain are also described.

La présente invention concerne une calpaïne de mammifère active du point de vue enzymatique, produite dans des cellules d'insectes par des techniques de recombinaison. On décrit des vecteurs de recombinaison et des baculovirus de recombinaison contenant l'ADN complémentaire codant la sous-unité de 80 kDa, la sous unité de 30 kDa ou les deux sous-unités. On décrit également des techniques de recombinaison pour produire de la calpaïne de mammifère active du point de vue enzymatique.

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