C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/17 (2006.01) C07K 14/00 (2006.01) C07K 14/62 (2006.01) C12N 15/62 (2006.01) C12N 15/63 (2006.01)
Patent
CA 2297382
The present invention provides a method of producing an insulin C-peptide, which comprises expressing in a host cell a multimeric polypeptide comprising multiple copies of a said insulin C-peptide, and cleaving said expressed polypeptide to release single copies of the insulin C-peptide. Also provided are nucleic acid molecules, expression vectors and host cells, for use in such a method and the multimeric insulin C-peptide polypeptide expressed and cleaved in such a method.
La présente invention concerne un procédé de production du peptide C de l'insuline, qui consiste à exprimer dans une cellule hôte un polypeptide multimère comprenant des copies multiples dudit peptide C, et à cliver ce polypeptide exprimé de manière à libérer des copies individuelles du peptide C de l'insuline. L'invention concerne également des molécules d'acide nucléique, des vecteurs d'expression et des cellules hôtes destinés à être utilisés lors du procédé précité, ainsi que le polypeptide multimère du peptide C de l'insuline exprimé et clivé conformément à ce même procédé.
Jonasson Per
Nygren Per-Ake
Stahl Stefan
Uhlen Mathias
Creative Peptides Sweden Ab
Fetherstonhaugh & Co.
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