Recombinant fusion proteines based on bacterial adhesines...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/62 (2006.01) C07K 14/16 (2006.01) C07K 14/245 (2006.01) C12N 15/31 (2006.01) G01N 33/531 (2006.01) G01N 33/555 (2006.01)

Patent

CA 2288348

The present invention relates to the field of biotechnology, particularly to the development of recombinant fusion proteines based on bacterial adhesines and their use in diagnostic assays of hemagglutination for the specific detection of antibodies or antigenes in the blood and other biological samples. The object from a technical point of view is to obtain recombinant fusion proteines, or biochemical conjugates, useful for the development of simple diagnostic assay sytems based on bacterial adhesines or their domains of molecular interaction with red cells. The use of adhesion proteines of E. coli as a base of fusion recombinant proteines with an activity of union to human erythrocytes enhances the characteristics of expression in said host thereby eliminating potential problems in production yields associated to bacterial toxicity.

L'invention concerne le domaine de la biotechnologie, plus particulièrement le développement de protéines de fusion recombinantes basées sur des adhésines bactériennes et leur utilisation dans les dosages diagnostiques d'hémagglutination afin de détecter spécifiquement des anticorps ou antigènes dans le sang ou dans d'autres essais biologiques. L'invention vise techniquement à obtenir des protéines de fusion recombinantes ou des conjugués biochimiques utiles pour le développement de systèmes simples de dosages diagnostiques basés sur des adhésines bactériennes et ses champs d'interaction moléculaire avec les globules rouges. L'utilisation de protéines d'adhésion de E. coli comme base de protéines recombinantes de fusion avec activité d'union à des érythrocytes humains favorise les caractéristiques d'expression dans cet hôte en éliminant des problèmes potentiels dans les rendements de production liés à la toxicité bactérienne.

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