Recombinant haemophilus influenzae protein and nucleotide...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/31 (2006.01) A61K 38/00 (2006.01) A61K 39/00 (2006.01) A61K 39/102 (2006.01) C07H 1/04 (2006.01) C07H 11/04 (2006.01) C07H 13/00 (2006.01) C07H 15/02 (2006.01) C07H 21/04 (2006.01) C07K 1/00 (2006.01) C07K 1/22 (2006.01) C07K 14/11 (2006.01) C07K 14/285 (2006.01) C07K 19/00 (2006.01) C12N 1/21 (2006.01) C12N 15/09 (2006.01) C12P 17/00 (2006.01) C12P 21/02 (2006.01) C12P 21/06 (2006.01) C12Q 1/34 (2006.01)

Patent

CA 2138765

Disclosed herein are immunogenic polysaccharide-H. influenzae adhesin protein conjugates, a purified H. influenzae adhesin protein and related proteins and polypeptides, DNA useful for producing the proteins, synthetic polyribosylribotol phosphate (PRP) oligosaccharides and intermediates useful for their synthesis, and methods of making and using these materials. The conjugates comprise a PRP fragment, preferably a synthetic oligosaccharide, coupled to an H. influenzae adhesin protein. The invention further comprises purified H. influenzae adhesin proteins and novel PRP oligosaccharides. The invention also comprises methods of producing these materials and using them in a vaccine to protect humans and other mammals against H. influenzae infection.

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