C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/55 (2006.01) C12N 1/15 (2006.01) C12N 1/21 (2006.01) C12N 9/14 (2006.01) C12N 11/00 (2006.01) C12N 15/70 (2006.01) C12P 7/04 (2006.01) C12P 7/16 (2006.01)
Patent
CA 2281931
The present invention is to haloaliphatic dehalogenase enzymes capable of converting halogenated aliphatic substrate molecules to vicinal halohydrins, as well as to DNA sequences encoding the polypeptide of the enzymes, to expression constructs containing this DNA, and to methods for producing the enzymes by placing the expression constructs into host cells under conditions sufficient for the transformants to produce the dehalogenase. A process for immobilizing the enzyme on a solid support and use of the immobilized enzyme for converting a halogenated aliphatic hydrocarbon to an alcohol is also disclosed.
L'invention concerne des déshalogénases haloaliphatiques capables de convertir les molécules de substrat aliphatique halogéné en halohydrines vicinales. L'invention concerne également des séquences d'ADN codant les polypeptides de ces enzymes, des produits d'expression recombinés renfermant cet ADN et des procédés pour élaborer les enzymes en introduisant lesdits produits dans des cellules hôtes, dans des conditions suffisantes pour que les transformants engendrent la déshalogénase. L'invention concerne en outre un procédé d'immobilisation de l'enzyme sur un support solide et l'utilisation de cette enzyme immobilisée pour convertir un hydrocarbure aliphatique halogéné en alcool.
Affholter Joseph A.
Kan Hueylin L.
Richard Ruth A.
Swanson Paul E.
Smart & Biggar
The Dow Chemical Company
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