Recombinant hepatitis a virus antigens obtained in plant cells

C - Chemistry – Metallurgy – 12 – N

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C12N 15/82 (2006.01) C07K 14/10 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2510482

The invention relates to recombinant hepatitis A virus antigens obtained in plant cells. More specifically, the invention relates to the generation of genetic constructions based on modified fragments of the genome of hepatitis A virus (HAV), using strain M2 which has been isolated in Cuba. The nucleotide sequences of said fragments fused with suitable positioning and regulation signals are expressed in transgenic plants, giving rise to recombinant HAV antigens comprising pentamers and/or empty coats, which can generate an immune response.

L'invention concerne des antigènes recombinés obtenus à partir de constructions génétiques basées sur des fragments modifiés du génome du virus de l'hépatite A (VHA) de la souche M2 isolée à Cuba. Les séquences nucléotidiques de ces fragments liées à des signaux de localisation et de régulation appropriés sont exprimées dans des plantes transgéniques, ce qui permet d'obtenir des antigènes recombinés du VHA, composés de pentomères et/ou d'enveloppes creuses, capables de produire une réponse immunitaire.

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