Recombinant herpesvirus of turkeys and uses thereof

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 7/01 (2006.01) A61K 39/17 (2006.01) A61K 39/255 (2006.01) A61K 39/265 (2006.01) A61K 39/295 (2006.01) C12N 15/19 (2006.01) C12N 15/38 (2006.01) C12N 15/45 (2006.01) C12N 15/50 (2006.01) C12N 15/86 (2006.01) C12N 15/869 (2006.01)

Patent

CA 2196570

This invention provides a recombinant herpesvirus of turkeys comprising a foreign DNA sequence encoding a cytokine inserted into an insertion region which comprises a XhoI site within an EcoR1 #9 fragment of a herpesvirus of turkeys viral genome, and the foreign DNA sequence encoding a cytokine which is capable of being expressed in a host cell infected with the herpesvirus of turkeys. This invention provides a recombinant herpesvirus of turkeys-Marek's disease virus chimera comprising a herpesvirus of turkeys unique long viral genome region and a Marek's disease virus unique short region. Lastly, this invention provides homology vectors for producing a recombinant herpesvirus of turkeys, host cells, and vaccines and methods for immunization.

Dans cette invention, on divulgue un virus herpétique recombinant de dindons comprenant une séquence d'ADN étrangère codant une cytokine insérée dans une région d'insertion contenant un site XhoI dans un fragment EcoR1 #9 d'un virus herpétique du génome viral du dindon, la séquence d'ADN étrangère codant une cytokine pouvant être exprimée dans une cellule hôte infectée par le virus l'herpétique du dindon. Cette invention porte également sur un virus herpétique recombinant d'une chimère d'un virus de la maladie de Marek des dindons comprenant un virus herpétique de région unique longue du génome viral des dindons et une région unique courte du virus de la maladie de Marek. Enfin, dans cette invention, on divulgue des vecteurs homologues pour la production d'un virus herpétique recombinant de dindon, des cellules hôtes, des vaccins et des méthodes d'immunisation.

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