Recombinant hexose oxidase, a method of producing same and...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/53 (2006.01) A21D 8/04 (2006.01) A23C 9/12 (2006.01) A23K 1/165 (2006.01) A23K 3/03 (2006.01) A23L 1/23 (2006.01) A23L 2/52 (2006.01) A23L 3/3571 (2006.01) A61K 8/66 (2006.01) A61K 31/70 (2006.01) A61K 38/44 (2006.01) A61Q 11/00 (2006.01) C12N 9/04 (2006.01) C12P 7/38 (2006.01) C12P 17/02 (2006.01) C12Q 1/26 (2006.01)

Patent

CA 2224143

A method of producing hexose oxidase by recombinant DNA technology, recombinant hexose oxidase and the use of such enzyme, in particular in the manufacturing of food products such as doughs and dairy products, animal feed, pharmaceuticals, cosmetics, dental care products and in the manufacturing of lactones. Suitable sources of DNA coding for the enzyme are marine algal species including Chondrus crispus, Iridophycus flaccidum and Euthora cristata. In useful embodiments, the recombinant hexose oxidase is produced by Pichia pastoris, Saccharomyces cerevisiae or E. Coli.

Procédé d'obtention d'hexose oxydase par génie génétique, hexose oxydase de recombinaison, et utilisation de cette enzyme, en particulier dans la fabrication de produits alimentaires, notamment pâtes levées et produits laitiers, d'aliments pour animaux, de produits pharmaceutiques, de cosmétiques, de produits d'hygiène dentaire et dans la fabrication de lactones. Certaines espèces d'algues marines, notamment Chondrus crispus, Iridophycus flaccidum et Euthora cristata sont des sources adéquates d'ADN codant cette enzyme. Selon des modes de réalisation intéressants, l'hexose oxydase de recombinaison est produite par Pichia pastoris, Saccharomyces cerevisiae ou E. coli.

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