Recombinant host for producing l-asparaginase ii

Details Recombinant host for producing l-asparaginase ii Recombinant host for producing l-asparaginase ii

C - Chemistry, Metallurgy

12

N

C12N 15/55 (2006.01) A61K 38/46 (2006.01) A61K 47/48 (2006.01) C12N 1/21 (2006.01) C12N 9/82 (2006.01) C12N 9/96 (2006.01) C12N 15/63 (2006.01) C12N 15/70 (2006.01)

Patent

CA 2652718

The invention provides a recombinant Escherichia coli host cell for producing an Escherichia coli L-asparaginase II enzyme. The host cell includes an Escherichia coli chromosome and at least one copy of a recombinant extrachromosomal vector, wherein the recombinant extrachromosomal vector encodes the L-asparaginase II enzyme, wherein the host cell chromosome also encodes the same L-asparaginase II enzyme, and wherein the host chromosome does not encode any other isoform of L-asparaginase II.

L'invention concerne l'utilisation d'une cellule hôte recombinante d'Escherichia coli pour l'obtention d'une enzyme L-asparaginase II d'Escherichia coli. Cette cellule hôte comprend un chromosome d'Eschericia coli et au moins une copie d'un vecteur extrachromosomique recombinant, vecteur qui code pour l'enzyme L-asparaginase II. Le chromosome de cellule hôte code également pour l'enzyme L-asparaginase II, mais ne code pas tout autre isoforme de L-asparaginase II.

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