Recombinant hosts suitable for simultaneous saccharification...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/56 (2006.01) C12N 1/21 (2006.01) C12N 9/24 (2006.01) C12N 15/67 (2006.01) C12N 15/70 (2006.01) C12P 7/06 (2006.01) C12P 7/10 (2006.01)

Patent

CA 2374524

The invention provides recombinant host cells containing at least one heterologous polynucleotide encoding a polysaccharase under the transcriptional control of a surrogate promoter capable of increasing the expression of the polysaccharase. In addition, the invention further provides such hosts with genes encoding secretory protein/s to facilitate the secretion of the expressed polysaccharase. Preferred hosts of the invention are ethanologenic and capable of carrying out simultaneous saccharification fermentation resulting in the production of ethanol from complex cellulose substrates.

L'invention concerne des cellules hôtes de recombinaison contenant au moins un polynucléotide hétérologue codant pour une polysaccharase sous le contrôle transcriptionnel d'un promoteur auxiliaire capable d'augmenter l'expression de la polysaccharase. De plus, l'invention concerne également des hôtes de ce type dotés de gènes codant pour une/des protéine(s) sécrétoire(s) permettant de faciliter la sécrétion de la polysaccharase exprimée. Selon l'invention, des hôtes préférés sont éthanologènes et capable de produire une saccharification et une fermentation simultanées se traduisant par la production d'éthanol à partir de substrats cellulosiques complexes.

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