Recombinant human factor viii derivative

Details Recombinant human factor viii derivative Recombinant human factor viii derivative

C - Chemistry, Metallurgy

12

N

C12N 15/62 (2006.01) C07K 14/755 (2006.01) C12N 9/64 (2006.01) C12N 15/57 (2006.01) C12N 15/85 (2006.01) C12P 21/02 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2071875

A DNA sequence coding for a biologically active recombinant human factor VIII derivative, comprising a first DNA segment coding for the 90 kDa chain of human factor VIII and a second DNA segment coding for the 80 kDa chain of human factor VIII; said segments being interconnected by a linker DNA segment coding for a linker peptide of 4 to about 100 amino acid residues of the B domain of human factor VIII, at least 4 of said amino acid residues originating from the C terminal of said domain; recombinant expression vector comprising such DNA sequence; host cells of animal origin transformed with such recombinant expression vector; a process for the manufacture of recombinant human factor VIII derivative; and human factor VIII derivative containing the heavy chain and the light chain. linked by metal ion bond.

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