Recombinant human factor viii derivatives

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/57 (2006.01) C07K 14/755 (2006.01) C12N 5/10 (2006.01) C12N 9/64 (2006.01)

Patent

CA 2081659

A DNA sequence coding for a biologically active recombinant human factor VIII derivative, comprising a first DNA seg- ment coding for the 90 kDa chain of human factor VIII and a second DNA segment coding for the 80 kDa chain of human fac- tor VIII, said segments being interconnected by a linker DNA segment coding for a linker peptide of 2 to 10 amino acid residues selected from lysine and arginine; recombinant expression vector comprising such DNA sequence; host cells of animal origin transformed with such recombinant expression vector; a process for the manufacture of recombinant human factor VIII deriva- tive; and human factor VIII derivative containing the heavy chain and the light chain linked by metal ion bon.

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