C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/14 (2006.01) A61K 38/38 (2006.01) C07K 1/36 (2006.01) C07K 14/765 (2006.01) A61K 38/00 (2006.01)
Patent
CA 2096572
ABSTRACT OF THE DISCLOSURE Human serum albumin obtained by gene manipulation techniques can be purified by a combination of specified steps in which a culture supernatant obtained from a human serum albumin-producing host is subjected to ultrafiltration, heat treatment, acid treatment and another ultrafiltration, followed by subsequent treatments with a cation exchanger, a hydrophobic chromatography carrier and an anion exchanger, and by salting- out to thereby obtain a pure form of human serum albumin which contains substantially no proteinous and polysaccharide contaminants, which is formulated into a pharmaceutical preparation. This process makes it possible to effeciently purify recombinant human serum albumin and to provide substantially pure human serum albumin which does not contain producer host-related substances and other contaminants and is sufficiently free from coloration. - 49 -
Fuluhata Naoto
Ishikawa Syoichi
Kamide Kaeko
Kondo Masahide
Noda Munehiro
Riches Mckenzie & Herbert Llp
Welfide Corporation
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