Recombinant intracellular pathogen vaccines and methods for use

A - Human Necessities – 61 – K

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A61K 39/02 (2006.01) A61K 39/002 (2006.01) A61K 39/04 (2006.01) A61K 39/116 (2006.01) A61P 31/02 (2006.01) A61P 31/04 (2006.01) A61P 33/02 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2406225

Vaccines and immunotherapeutics for preventing intracellular pathogen diseases in mammals are provided that consist of recombinant attenuated intracellular pathogens that have been transformed to express recombinant immunogenic antigens of the same r other intracellular pathogens. Exemplary vaccines and immunotherapeutics include attenuated recombinant Mycobacteria expressing the major extracellular non-fusion proteins of Mycobacterial and/or other intracellular pathogens. These exemplary vaccines are shown to produce surprisingly potent protective immune response in mammals that surpass those of any previously known anti-mycobacterium vaccine. More specifically, a recombinant BCG expressing the 30 kDa major extracellular non-fusion protein of Mycobacterium tubercolosis is provided. Additionally, methods for preventing and treating diseases caused by intracellular pathogens are provided. The methods of treating and preventing intracellular pathogen diseases utilize the described surprisingly efficacious vaccines and immunotherapeutics.

L'invention concerne des vaccins et des immunothérapies servant à empêcher des maladies dues à des agents pathogènes intracellulaires chez les mammifères. Ces vaccins et immunothérapies font appel à des agents pathogènes intracellulaires atténués recombinants qui ont été transformés pour exprimer des antigènes immunogènes recombinants de ceux-ci ou d'autres agents pathogènes intracellulaires. Ces vaccins et immunothérapies exemplaires comprennent notamment des mycobactéries exprimant les principales protéines non hybrides extracellulaires des mycobactéries et/ou d'autres agents pathogènes intracellulaires. Ces vaccins exemplaires produisent des réponses immunitaires de protection étonnamment puissantes chez les mammifères, et qui surpassent celles de n'importe quel vaccin anti-mycobactérium connu précédemment. Plus spécifiquement, l'invention concerne un bacille bilié de Calmette Guérin (BCG) recombinant, exprimant la principale protéine non hybride extracellulaire 30 kDa de Mycobacterium tubercolosis. L'invention concerne également des procédés servant à empêcher et à traiter des maladies causées par des agents pathogènes intracellulaires. Ces procédés servant à traiter et à empêcher les maladies pathogènes intracellulaires font appel aux vaccins et immunothérapies étonnamment efficaces décrits.

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