Recombinant kat enzyme and process for its preparation

Details Recombinant kat enzyme and process for its preparation Recombinant kat enzyme and process for its preparation

C - Chemistry, Metallurgy

12

N

C12N 15/54 (2006.01) A61K 31/70 (2006.01) A61K 35/12 (2006.01) A61K 48/00 (2006.01) C07H 21/00 (2006.01) C12N 9/10 (2006.01) C12P 17/12 (2006.01) C12Q 1/02 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2194447

Disclosed are isolated DNAs encoding a kynurenine aminotransferase selected from the group consisting of: (a) isolated DNA sequences which encode rat KAT; (b) an isolated DNA sequence which hybridizes to isolated DNA sequences of (a) above and which encodes a mammalian KAT enzyme; and (c) an isolated DNA sequence differing from the isolated DNA sequences of (a) and (b) above in codon sequence due to the degeneracy of the genetic code, and which encodes a KAT enzyme. Vectors and host cells containing the same, oligonucleotide probes for identifying kynurenine aminotransferase, and isolated and purified kynurenine aminotransferase are also disclosed.

L'invention porte sur des ADN isolés codant pour une kynurénine aminotransférase (KAT) sélectionnée dans le groupe comprenant: (a) des séquences isolées d'ADN codant pour la KAT du rat; (b) une séquence isolée d'ADN s'hybridant à des séquences de type (a) et codant pour une enzyme KAT d'un mammifère; et (c) une séquence isolée d'ADN différant des séquences (a) et (b) ci-dessus dans leur séquence de codons en raison de la dégénérescence de leur code génétique et qui code pour une enzyme KAT. L'invention porte également sur des cellules vecteurs et des cellules hôtes les contenant sur des sondes oligonucléotidiques permettant d'identifier la kynurénine aminotransférase, et sur la kynurénine aminotransférase isolée et purifiée.

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