C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/74 (2006.01) A23C 9/12 (2006.01) A23C 9/123 (2006.01) A23K 3/00 (2006.01) A23L 1/03 (2006.01) A23L 3/3571 (2006.01) A61K 35/74 (2006.01) C12N 1/21 (2006.01) C12N 15/09 (2006.01) C12N 15/11 (2006.01) C12N 15/77 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2152898
A method of isolating a lactic acid bacterial DNA fragment comprising a promotor is provided. The method involves introduction of a DNA molecule, containing a transposable element comprising a promotorless structural gene as a promotor probe gene, into a population of a lactic acid bacterium. The invention pertains to construction of a recombinant lactic acid bacterium comprising a regulatable promotor by using the above method. A recombinant lactic acid bacterium comprising a gene coding for a desired gene product and operably linked thereto a regulatable lactic acid bacterial promotor not natively associated with the gene are further provided. The invention as described relates to the use of such a recombinant lactic acid bacterium and DNA constructions comprising a regulatable lactic acid bacterial promotor.
Hansen Egon Bech
Israelsen Hans
Johansen Eric
Madsen Soren Michael
Nilsson Dan
Bioneer A/s
Bioteknologisk Institut
Blake Cassels & Graydon Llp
Chr. Hansen A/s
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