Recombinant lysostaphin analogs

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/57 (2006.01) A61K 38/48 (2006.01) A61K 48/00 (2006.01) C12N 9/52 (2006.01) C12N 15/87 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2305624

The present invention relates to lysostaphin analogs having a molecular structure preventing post-translational modifications while retaining lysostaphin-like biological activity. Such recombinant lysostaphin analogs have at least one modified site, or a rearrangement of at least one site at the level of the amino acid sequence of the mature lysostaphin protein. The invention also relates to DNA sequences encoding the lysostaphin analogs, and recombinant plasmids and host cells for the expression of recombinant lysostaphin analog.

La présente invention a trait à des analogues de lysostaphine possédant une structure moléculaire empêchant les modifications intervenant après la traduction, tout en maintenant l'activité biologique du type lysostaphine. De tels analogues recombinants de lysostaphine présentent au moins un site modifié, ou un réagencement d'au moins un site au niveau de la séquence d'acides aminés de la protéine mature de lysostaphine. Cette invention concerne également des séquences d'ADN codant ces analogues de lysostaphine, ainsi que des plasmides recombinants et des cellules hôtes permettant d'exprimer un analogue de lysostaphine recombinant.

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