Recombinant lysostaphin analogs

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/57 (2006.01) A61K 38/48 (2006.01) C12N 9/52 (2006.01) C12N 15/87 (2006.01) A61K 38/00 (2006.01) A61K 48/00 (2006.01)

Patent

CA 2211293

The present invention relates to lysostaphin analogs having a molecular structure preventing post-translational modifications while retaining lysostaphin-like biological activity. Such recombinant lysostaphin analogs have at least one modified site, or a rearrangement of at least one site at the level of the amino acid sequence of the mature lysostaphin protein. The invention also relates to DNA sequences encoding the lysostaphin analogs, and recombinant plasmids and host cells for the expression of recombinant lysostaphin analog.

La présente invention porte sur des analogues de la lysostaphine possédant une structure moléculaire qui empêche toute modification post-traductionnelle, tout en conservant l'activité biologique de type lysostaphine. Ce type d'analogues de lysostaphine recombinants possède au moins un site modifié ou un réarrangement d'au moins un site au niveau de la séquence d'aminoacides de la protéine de lysostaphine mature. L'invention porte également sur des séquences d'ADN codant les analogues de lysostaphine, ainsi que sur des plasmides recombinants et des cellules hôtes pour l'expression des analogues de lysostaphine recombinants.

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