Recombinant materials and methods for producing human...

Details Recombinant materials and methods for producing human... Recombinant materials and methods for producing human...

C - Chemistry, Metallurgy

12

P

530/13, 195/1.18

C12P 21/00 (2006.01) C07H 15/12 (2006.01) C07K 14/245 (2006.01) C07K 14/575 (2006.01) C12N 1/20 (2006.01) C12N 15/70 (2006.01) A61K 38/00 (2006.01)

Patent

CA 1275953

RECOMBINANT MATERIALS AND METHODS FOR PRODUCING HUMAN CONNECTIVE TISSUE-ACTIVATING PEPTIDE-III AND ANALOGS THEREOF Abstract A synthetic structural gene encoding CTAP-III or CTAP-III-Leu21 including adaptors for the carboxy and amino terminal ends of the gene which contain start and stop codons and convenient restriction sites for use in cloning the gene is described. The gene was designed for efficient expression in bacteria and to include two unique restriction sites for BamHI and XbaI. Plasmid expression vectors that are derivatives of pBR322 that contain a ColEl insert which includes the expression control sequence and structural gene for colicin are also described. Constructs for expressing CTAP-III, CTAP-III- Leu21, CTAP-III or CTAP-III-Leu21 having a nonpolar pentapeptide fused to its amino terminus, and fusion proteins of such CTAP-III proteins and a colicin fragment are prepared by inserting the synthetic structural gene into the vectors at positions in phase with the colicin expression control sequence and under the control thereof.

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