Recombinant microorganisms capable of fermenting cellobiose

Details Recombinant microorganisms capable of fermenting cellobiose Recombinant microorganisms capable of fermenting cellobiose

C - Chemistry, Metallurgy

12

N

C12N 15/54 (2006.01) C12N 1/20 (2006.01) C12N 9/12 (2006.01) C12N 9/42 (2006.01) C12N 15/01 (2006.01) C12N 15/55 (2006.01) C12P 7/06 (2006.01) C12P 7/10 (2006.01)

Patent

CA 2285670

This invention relates to a recombinant microorganism which expresses pyruvate decarboxylase, alcohol dehydrogenase, Klebsiella phospho-.beta.-glucosidase and Klebsiella (phosphoenolpyruvate-dependent phosphotransferase system) cellobiose-utilizing Enzyme II, wherein said phospho-.beta.-glucosidase and said (phosphoenolpyruvate- dependent phosphotransferase) cellobiose-utilizing Enzyme II are heterologous to said microorganism and wherein said microorganism is capable of utilizing both hemicellulose and cellulose hydrolysates, including cellobiose, in the production of ethanol.

Micro-organisme de recombinaison qui exprime la pyruvate décarboxylase, l'alcool-déshydrogénase, la phospho- beta -glucosidase de Klebsiella et l'enzyme II de Klebsiella utilisant la cellobiose (système de phosphotransférase phosphoenolpyruvate-dépendante). La phospho- beta -glucosidase et l'enzyme II utilisant la cellobiose (système de phosphotransférase phosphoenolpyruvate-dépendante) sont hétérologues pour ce micro-organisme et ce micro-organisme est capable d'utiliser aussi bien les hydrolysats d'hémicellulose que de cellulose, y compris la cellobiose, pour la production d'éthanol.

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