Recombinant nonstructural protein subunit vaccine against...

A - Human Necessities – 61 – K

Patent

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A61K 39/12 (2006.01) A61K 39/395 (2006.01) C07K 14/18 (2006.01) C12N 15/40 (2006.01)

Patent

CA 2340788

The recombinant expression and secretion from eucaryotic host cells, particularly Drosophila cells, of Flavivirus nonstructural (NS) protein, particularly NS1, is useful in combination with Flavivirus truncated envelope (E) protein to protect a host subject from infection and disease from Flavivirus species. Further, NS1 is useful as a diagnostic of flaviviral infection. Compositions of truncated flaviviral envelope protein and flaviviral nonstructural protein induce high titer virus neutralizing antibodies believed to be important in protection against flaviviral infection and which are useful in diagnosis of infection by the virus.

L'invention concerne l'expression recombinante et la sécrétion par des cellules hôtes eucaryotes, en particulier par des cellules de drosophile, d'une protéine non structurale (NS) tronquée de Flavivirus, en particulier de NS1. Combinée à une protéine d'enveloppe (E) tronquée de Flavivirus cette protéine structurale permet de protéger un sujet hôte contre l'infection et les maladies causées par Flavivirus. NS1 est en outre utile pour diagnostiquer une infection flavivirale. L'invention concerne également des compositions comprenant la protéine d'enveloppe tronquée de Flavivirus et la protéine non structurale de Flavivirus, et qui entraînent un titre élevé d'anticorps neutralisants considérés comme importants dans la protection contre l'infection flavivirale, ainsi que des compositions utiles pour le diagnostic d'une infection par ce virus.

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