Recombinant north american type 1 porcine reproductive and...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 7/04 (2006.01) C07K 14/08 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2691755

Porcine reproductive and respiratory syndrome virus (PRRSV) is a major problem in the pork industry worldwide. The inclusion of markers in vaccines will allow for diagnostic differentiation of vaccinated animals from those naturally infected with wild-type virus. Using a cDNA infectious clone of North American Type 1 PRRSV, a recombinant green fluorescent protein (GFP) tagged PRRSV has been made, containing deletion of an immunogenic epitope, ES4, in the nsp2 region. GFP and ES4 epitope-based ELISAs compliment the marker identification.

Le virus du syndrome dysgénésique et respiratoire porcin (VSDRP) est un problème majeur dans l'industrie porcine au niveau mondial. L'inclusion de marqueurs dans des vaccins permet d'effectuer une différenciation diagnostique entre les animaux vaccinés et ceux naturellement infectés par le virus de type sauvage. Au moyen d'un clone infectieux d'ADNc du VSDRP de type 1 nord-américain, un VSDRP marqué par la protéine verte fluorescente (GFP) recombinant a été réalisé, ce virus présentant une délétion d'un épitope immunogénique, ES4, dans la région nsp2. Des essais ELISA reposant sur la GFP et sur l'épitope ES4 complètent l'identification de marqueurs.

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