Recombinant nucleic acid sequences and methods for...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/70 (2006.01) C12N 1/21 (2006.01) C12N 15/53 (2006.01) C12N 15/74 (2006.01) C12Q 1/18 (2006.01) C12Q 1/66 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2252699

A recombinant nucleic acid sequence comprising an SOS regulated promoter with an induction ratio higher than 40, said promoter being operatively linked to a reporter encoding nucleic acid sequence encoding a reporter resulting in a signal that can be assayed as light production. A biosensor comprising such a nucleic acid sequence and methods for determining genotoxicity and the presence of multiple genotoxic compounds using such a biosensor are disclosed.

Cette séquence d'acides nucléiques recombinée comprend un promoteur régulé SOS, lequel présente un rapport d'induction supérieur à 40, est lié de façon fonctionnelle à une séquence d'acides nucléiques codant un marqueur, lequel codage produit un signal pouvant être analysé en tant que production de lumière. On décrit également un biocapteur comprenant une telle séquence d'acides nucléiques, ainsi que des procédés de détermination de la génotoxicité et de la présence de plusieurs composés génotoxiques, à l'aide d'un tel biocapteur.

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