Recombinant plants mimicking the activity of the hp-1 mutant...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/29 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) C07K 14/415 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2429686

A method for increasing the phytonutrient and/or chlorophyll content and/or yield of a crop, said method comprising transforming a plant cell from which viable plants may be recovered, with a DNA construct comprising a sequence which encodes phytochrome A or a moiety which interacts with the phytochrome A pathway so as to mimic the activity of the hp-1 mutant of tomato, said sequence being under the control of a tissue specific promoter which is specific for a crop tissue of the plant, and thereafter generating viable plants from said cell. Various means of achieving this are described.

L'invention se rapporte à un procédé permettant d'accroître la teneur en éléments phytonutritifs et/ou en chlorophylle, et/ou le rendement d'une culture. Ce procédé consiste à transformer une cellule végétale, à partir de laquelle des plantes viables peuvent être récupérées, lesquelles sont pourvues d'une construction d'ADN comportant une séquence codant un phytochrome A, ou une fraction agissant avec la voie du phytochrome A de façon à imiter l'activité du mutant hp-1 de la tomate. Cette séquence se trouve sous le contrôle d'un promoteur spécifique à un tissu qui est spécifique à un tissu de culture de la plante. Le procédé consiste ensuite à générer des plantes viables à partir de ladite cellule. L'invention porte également sur différents moyens d'y parvenir.

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