Recombinant plasmid for the expression of l-phenylalanine...

Details Recombinant plasmid for the expression of l-phenylalanine... Recombinant plasmid for the expression of l-phenylalanine...

C - Chemistry, Metallurgy

12

N

195/1.2, 195/1.3

C12N 15/60 (2006.01) C12N 1/20 (2006.01) C12N 9/88 (2006.01) C12N 15/70 (2006.01)

Patent

CA 1293461

- 55 - Abstract of the Disclosure A recombinant plasmid fox the expression of phenylalanine ammonia-lyase (PAL) is constructed by incorporating therein a combined promoter comprising (a) the fusion promoter (the tac promoter) composed of the trp promoter minus 35 region and the lac UV-5 promoter minus 10 region and (b) the PL promoter of the lambda phage, the tac promoter and the PL promoter being connected so as to have the same directional property. This recombinant plasmid permits more efficient expression of PAL in Escherichia coli.

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