Recombinant plasmid inserted with herpes simplex virus gene

Details Recombinant plasmid inserted with herpes simplex virus gene Recombinant plasmid inserted with herpes simplex virus gene

C - Chemistry, Metallurgy

12

N

195/1.2

C12N 15/38 (2006.01) A61K 39/245 (2006.01) C07K 14/035 (2006.01) C12N 15/81 (2006.01) C12P 21/08 (2006.01) A61K 39/00 (2006.01)

Patent

CA 1295564

Abstract: The invention provides a recombinant plasmid inserted with a herpes simplex virus gene. The plasmid comprises a plasmid vector containing a yeast gene and an Escherichia coli gene and carries a promoter region and a herpes simplex virus gB gene (HSVgB gene) recombined therewith under control of the promoter. The HSVgB gene omits a part of the N-terminal coding region of the gene includ- ing the signal sequence-encoding region and optionally a further region downstream therefrom, e.g. a gB gene omitting a gene encoding the N-terminal 30 amino acids, and a gB gene omitting a gene encoding the N-terminal 83 amino acids. The recombinant plasmid is useful for the production of a transformed yeast, which is useful for the production of HSVgB proteins suitable for the production of HSV vaccine and diagnostic reagents for herpes simplex virus infections.

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