Recombinant plasmid psp1, microorganisms transformed...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/63 (2006.01) C12N 9/52 (2006.01) C12N 15/57 (2006.01) C12N 15/68 (2006.01) C12N 15/74 (2006.01)

Patent

CA 2415265

The invention relates to a recombinant plasmid pSP1 harboring the gene vapk, which encodes alkaline protease VapK, and par gene, which is associated with the stability of plasmid, a microorganism Vibrio metschnikovii transformed therewith, and method for producing an alkaline protease VapK using the same microorganism.

L'invention concerne un plasmide recombiné pSP1 logeant le gène <i>vapk</i>, lequel code pour la protéase alcaline VapK, ainsi que le gène <i>par</i>, lequel est associé à la stabilité du plasmide; elle concerne également un micro-organisme Vibrio metschinikovii transformé à l'aide de ce plasmide, ainsi qu'un procédé de production d'une protéase alcaline VapK à l'aide de ce micro-organisme.

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