Recombinant plasmodium falciparum merozoite protein-1/42...

A - Human Necessities – 61 – K

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A61K 39/015 (2006.01) A61P 33/06 (2006.01) C07K 14/445 (2006.01) G01N 33/569 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2462946

In this application is the expression and purification of a recombinant Plasmodium falciparum (3D7) MSP-142. The method of the present invention produces a highly purified protein which retains folding and disulfide bridging of the native molecule. The recombinant MSP-142 is useful as a diagnostic reagent, for use in antibody production, and as a vaccine.

La présente invention concerne l'expression et la purification d'un précurseur de recombinaison MSP-1¿42? de Plasmodium falciparum (3D7). Le procédé selon l'invention permet de produire une protéine fortement purifiée qui conserve le repliement et la formation de liaison disulfure de la molécule native. Le précurseur MSP-1¿42? est utile en tant que réactif de diagnostic, qu'on peut employer dans la production d'anticorps et en tant que vaccin.

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