Recombinant platelet collagen receptor glycoprotein vi and...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/12 (2006.01) A61K 38/17 (2006.01) C07K 14/705 (2006.01) G01N 33/68 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2372515

The invention relates to Glycoprotein VI (GPVI), its isolation, purification, and methods for recombinant production. Especially, the invention relates to the use of GPVI, preferably recombinant GPVI, in the treatment of disorders and pathological events correlated directly or indirectly to blood coagulation disorders such as thrombotic and cardiovascular diseases. The extracellular recombinant protein can also be used for establishing screening assays to find potential inhibitors of the membrane bound GPVI in order to inhibit binding of thrombocytes and platelets, respectively, to collagen. Changes in GPIV can be used to monitor platelet age and exposure to thrombotic and cardiovascular diseases.

L'invention concerne la glycoprotéine VI (GPVI), son isolation, sa purification et des procédés de production par reconstruction. En particulier, l'invention a pour objet l'utilisation de la GPVI, de préférence la GPVI de reconstruction, dans le traitement de troubles et de problèmes pathologiques liés directement ou indirectement aux troubles de la coagulation sanguine, telles que les maladies cardiovasculaires et les thromboses. La protéine de reconstruction extracellulaire peut également être utilisée dans des dosages pour détecter des inhibiteurs potentiels de la GPVI liée à la membrane pour inhiber la liaison des thrombocytes et des plaquettes, respectivement, au collagène. Les modifications de la GPVI permettent de contrôler l'age des plaquettes et l'exposition aux maladies cardiovasculaires et aux thromboses.

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